Tag-arkiv: Karolinska Institutet

Postdoctoral Position in Immune-targeting of Leukemic Stem Cells

Postdoctoral Position in Immune-targeting of Leukemic Stem Cells

Karolinska Institutet, the Department of Medicine, Huddinge

Dept. of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden
We are looking for highly motivated and talented postdoctoral candidate to take the lead on exciting research projects in the lab of Professor Sten Eirik Jacobsen (https://ki.se/en/medh/sten-eirik-w-jacobsen-lab-hematopoietic-stem-cell-biology-group) and Assistant Professor Petter S. Woll (https://ki.se/en/medh/petter-woll-group). The position and corresponding research project are focused on immune-targeting of leukemic stem cells using genetically-modified T cell receptors recognizing antigens expressed on leukemic stem cells. The project is funded by existing grants, including from the Knut and Alice Wallenberg Foundation.

About the research project
The Jacobsen and Woll labs comprises an international collaborative team focused on understanding at the single cell level the cellular and molecular pathways governing the earliest fate decisions in normal hematopoiesis [1-4] and how these are dysregulated in hematologic malignancies [5-9]. Our recent identification of distinct leukemic stem cells in patients with myelodysplastic syndromes and the demonstration that these selectively escape therapy resulting in relapse, highlight the need for development of targeted strategies that allow the elimination rare leukemic stem cells. In collaboration with Professor Johanna Olweus at the University of Oslo (https://www.ous-research.no/olweus/) who has developed novel strategies to exploit the specificity and potency of CD8 T cells expressing antigen-specific T cell receptors (TCRs) for cancer therapy [10, 11], we have identified candidate antigens expressed in leukemic stem cells that we now will seek to target experimentally.

This project will be pursued in part at the Center for Hematology and Regenerative Medicine (HERM; http://ki.se/en/medh/herm), and in part at the Department of Cell and Molecular Biology (CMB; http://ki.se/en/cmb/startpage). The Jacobsen and Woll labs also have close interactions with other world leading research groups, and the recruited postdoctoral candidate will benefit from interactions in this highly collaborative network.

Responsibilities
The postholder will take the lead, together with the group leaders and collaborators, to actively pursue novel studies on cellular and molecular mechanism that allows efficient immune-targeting of leukemic stem cells. This is studied in mouse models, as well as in relevant patients. Key technologies applied towards these studies include flow cytometry, mouse genetics, in vitro and in vivo analysis of stem cell and immune cell activity, and high-throughput DNA and RNA sequencing including single cell analysis. The postholder must enjoy team work and interact well with others, including other members of lab, as well as collaborating scientists and clinicians. Good communication skills will also be required for data presentations and to represent the group at local or external conferences.

Essential requirements

  • PhD degree, or PhD students scheduled to defend within three months after the application deadline, in which the latter require case verification from the Dissertation Committee (or equivalent body)
  • Strong track record as evidenced by one or more high impact publications in relevant research fields
  • Training in international leading laboratories
  • Good knowledge of spoken and written English
  • Excellent interpersonal and communication skills
  • Demonstrate ability to organize, plan and document experimental bench-work
  • Ability to stay on top of the relevant research field 

Desired requirements

  • Experience with advanced state-of-the art flow cytometry
  • Experience with mouse work
  • Experience with next-generation sequencing analysis including RNA and DNA seq, ChIPseq, HiC, and similar assays as well as bioinformatics analysis and computational modeling based on such data
  • Experience with molecular analysis of small cell numbers, including single cell analysis

Application process
An employment application must contain the following documents in English or Swedish:

  1. A maximum one page cover letter summarizing motivation for the position, previous experience and research interest
  2. A complete curriculum vitae of current work and work history
  3. A complete list of publications
  4. Names and contact details of three references who are familiar with the applicant’s recent research and training

 References

  1. Nature, 2018. 554(7690): p. 106-111.
  2. Nature, 2013. 502(7470): p. 232-6.
  3. Nat Immunol, 2016. 17(12): p. 1424-1435.
  4. Cell Stem Cell, 2013. 13(5): p. 535-48.
  5. Cancer Cell, 2018. 33(2): p. 274-291 e8.
  6. Nat Med, 2017. 23(6): p.692-702.
  7. Cancer Cell, 2014. 25(6): p. 794-808.
  8. N Engl J Med, 2012. 366(10): p. 967-9.
  9. N Engl J Med, 2010. 363(11): p. 1025-37.
  10. Nat Protoc, 2019. 14(6): p. 1926-1943.
  11. Science, 2016. 352(6291): p. 1337-41.

Location: Flemingsberg

The application is to be submitted through the Varbi recruitment system.

Want to make a difference? Join us and contribute to better health for all

Karolinska Institutet is one of the world’s leading medical universities. Its vision is to significantly contribute to the improvement of human health. Karolinska Institutet accounts for the single largest share of all academic medical research conducted in Sweden and offers the country’s broadest range of education in medicine and health sciences. The Nobel Assembly at Karolinska Institutet selects the Nobel laureates in Physiology or Medicine.
Pursuant to the regulations of the Swedish National Archives, applications are kept on file for two years after the appointment has gained legal force. The regulations do not apply to attachments that have been printed or otherwise published.
Karolinska Institutet strives to provide a workplace that has approximately the same number of women and men, is free of discrimination and offers equal opportunity to everyone.
For temp agencies and recruiters, and to salespersons: We politely, yet firmly, decline direct contact with temp agencies and recruiters, as well as those selling additional job announcements.

Type of employmentTemporary position longer than 6 months
Contract typeFull time
First day of employmentAccording to agreement
Number of positions1
Working hours100%
CityHuddinge
CountyStockholms län
CountrySweden
Reference number2-5384/2019
ContactPetter Woll, Petter.woll@ki.se
Union representativeCarina Eklund, OFR, Carina.eklund@ki.seBelinda Pannagel, SACO, Belinda.pannagel@ki.se
Last application date28.Nov.2019 11:59 PM CET

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Post expires on Thursday November 28th, 2019

Postdoctoral studies in Cancer Metabolism (scholarship)

Karolinska Institutet, institutionen för fysiologi och farmakologi

At the Department of Physiology and Pharmacology we teach and conduct internationally acclaimed, high-quality research in Physiology, Pharmacology, Anesthesiology and Intensive care. We have around 400 employees, 30 research groups, and 90 doctoral students. Our research fields include cellular signaling, cardiovascular and respiratory systems, neuropsychopharmacology, neuroinflammation, pharmacogenetics, regenerative medicine, Renal System, anesthesiology and intensive care, muscle physiology and pain. Our collaboration with the clinics at Karolinska University Hospital provides unique opportunities for translational research within our subjects. More information about research at http://ki.se/fyfa_english

Research Group and Project Description
Tumor cells display metabolic profiles that are remarkably distinct from their normal tissue of origin. This is to ensure fulfillment of their higher bioenergetic/biosynthetic needs required to support rapid proliferation.
Tumor cells display metabolic profiles that are remarkably distinct from their normal tissue of origin. This is to ensure fulfillment of their higher bioenergetic/biosynthetic needs required to support rapid proliferation. Less is known about metabolic heterogeneity in seemingly identical cancer cells. We have previously identified metabolic heterogeneity in lymphomas that can be rationally targeted (Cancer Cell. 2012, Cell Death Differentiation. 2017) and more recently in lung adenocarcinomas (Cell Reports, 2017, Journal of Cancer, 2017). Our group focuses on understanding fundamental mechanisms underlying metabolic heterogeneity. Hence, we are currently trying to identify molecular regulators that shape the tumor metabolism and metabolic diversity, with the ultimate goal of identifying novel drug targets and biomarkers.  
For more information please see our published work:

Cell Reports 2017 Jun 13;19(11):2289-2303)

Journal of Cancer 2017;8(11):2088)

Cell Death Differentiation. 2017 Feb;24(2):251-262 .

Cancer Cell 2012 Oct;22(4):547-60.

Duties
The candidate will conduct research within the field of biochemical cancer research with a focus on tumor metabolism. We seek exceptional, dedicated, self-motivated and hardworking individuals with a positive attitude and with substantial experience in molecular biology or biochemistry or cell biology. The candidate should have a strong background in cancer biology and have a strong track record (as shown by first-author publications in international peer-reviewed journals).  

Entry requirements
Scholarships for postdoctoral qualification can be established for foreign researchers who place their qualifications in Sweden. The purpose of scholarships for postdoctoral qualification is to promote internationalization and contribute to research qualification after a doctorate or equivalent.
A scholarship for carrying out postdoctoral research can be granted for a maximum of two years within a four year period following the receipt of a doctoral degree or equivalent.
To be eligible for a postdoctoral scholarship, the person must have obtained a doctorate or a foreign degree deemed to be equivalent to a doctorate.

The head of the department determines whether their previous training and scholarly qualifications correspond to a Swedish doctorate or higher.

Other requirements
The candidate must have hands-on experience with:

  • – Mammalian cell culturing techniques incl. siRNA/overexpression techniques.
  • – SDS-PAGE, Western blot, Quantitative PCR

Desired skills

  • – Experience using an Extracellular Flux Analyzer (Seahorse technology)
  • – Flow cytometry and Confocal and imaging techniques
  • – Experience in proteomics and/or metabolomics.

The candidate should be able to thrive and deliver results on goals in a fast-paced, dynamic and highly multi-tasking environment. The candidate is expected to contribute to scientific discussions and presenting outcomes to colleagues beyond maintaining detailed and organized records of project data and other materials as needed. Strong verbal and written communication skills in English are required.

Type of scholarship
The amount is tax free and it is set for twelve months at a time, paid out on a six months basis. In exceptional cases, shorter periods may be acceptable.

Application process
An application must contain the following documents in English or Swedish:

  • A complete curriculum vitae, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work
  • A complete list of publications
  • A summary of current work (no more than one page)
  • Uploaded Reference letters from, at least, two previous employers with contact information.

The application is to be submitted on the Varbi recruitment system.

Karolinska Institutet is one of the world’s leading medical universities. Its vision is to significantly contribute to the improvement of human health. Karolinska Institutet accounts for the single largest share of all academic medical research conducted in Sweden and offers the country’s broadest range of education in medicine and health sciences. The Nobel Assembly at Karolinska Institutet selects the Nobel laureates in Physiology or Medicine.

Pursuant to the regulations of the Swedish National Archives, applications are kept on file for two years after the appointment has gained legal force. The regulations do not apply to attachments that have been printed or otherwise published.

Karolinska Institutet strives to provide a workplace that has approximately the same number of women and men, is free of discrimination and offers equal opportunity to everyone.
For temp agencies and recruiters, and to salespersons: We politely, yet firmly, decline direct contact with temp agencies and recruiters, as well as those selling additional job announcements.

Reference number2-5065/2019
ContactErik Norberg, erik.norberg@ki.se
Union representative Göran Engberg, SACO, 08-524 867 17Henry Wölling, SEKO, 08-524 840 80Niklas Andersson, OFR, niklas.andersson@ki.se
Last application date24.Nov.2019 11:59 PM CET

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Post expires on Sunday November 24th, 2019

Postdoctoral position in Epithelial Stem Cell Biology

Karolinska Institutet, Institutionen för cell- och molekylärbiologi, Genander

The Department of Cell and Molecular Biology (CMB) is a department with a strong focus on basic science. CMB conducts research and education in cell biology, molecular biology, developmental biology, stem cell biology, cancer and infection biology.

Focus of the lab
The postdoctoral project will be conducted in Maria Genander’s research group and is funded by ERC. More information about the Genander lab can be found here: http://ki.se/en/cmb/maria-genanders-group

The Genander lab is interested in understanding establishment and maintenance of epithelial tissue homeostasis and how alterations in homeostasis lead to tumor formation. Specifically, we are interested in identifying and mechanistically delineate stem cell niche components required for normal stem cell properties and how these stem cell-to-niche interactions change during tumor progression.


Project description
The project is focused on identifying esophageal stem cell niche components required for esophageal homeostasis. We aim use single cell RNA-Sequencing to identify subpopulation of stromal cells in mouse and human esophagus influencing normal tissue homeostasis. Furthermore, we aim to delineate the dynamics of these subpopulations during tumor formation and progression. Functional verification of specific subpopulations will be done using transgenic mouse models.

Entry requirements
We are looking for a highly motivated candidate with a PhD in Biomedicine or related area. We expect the candidate to have a strong scientific background with relevant expertise and publications. Previous experience working with mouse models is a requirement. Previous experience of stem cell/tumor biology or gene expression analysis is a strength. The applicant is expected to have expertise in basic cell and molecular methods. The working language is English, and the applicant is expected to be proficient in both spoken and written English. 


Application process
An employment application must contain the following documents in English or Swedish:

  • A complete curriculum vitae, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work
  • A complete list of publications
  • Contact information of two references, recomendation letters are also welcome
  • A brief letter of motivation (no more than one page)
  • A summary of current work (no more than one page)

The application is to be submitted through the Varbi recruitment system.

Karolinska Institutet is one of the world’s leading medical universities. Its vision is to significantly contribute to the improvement of human health. Karolinska Institutet accounts for the single largest share of all academic medical research conducted in Sweden and offers the country’s broadest range of education in medicine and health sciences. The Nobel Assembly at Karolinska Institutet selects the Nobel laureates in Physiology or Medicine.
Pursuant to the regulations of the Swedish National Archives, applications are kept on file for two years after the appointment has gained legal force. The regulations do not apply to attachments that have been printed or otherwise published.
Karolinska Institutet strives to provide a workplace that has approximately the same number of women and men, is free of discrimination and offers equal opportunity to everyone.
For temp agencies and recruiters, and to salespersons: We politely, yet firmly, decline direct contact with temp agencies and recruiters, as well as those selling additional job announcements.

Type of employmentTemporary position longer than 6 months
Contract typeFull time
First day of employmentAs soon as possible
Number of positions1
Working hours100%
CityStockholm
CountyStockholms län
CountrySweden
Reference number2-4690/2019
ContactMaria Genander, maria.genander@ki.se
Union representativeBiborka Bereczky Veress, SACO, 46 70-173 85 75Elisabeth Valenzuela, SEKO, 08-524 863 53Magnus Anå, OFR, 08-524 864 37, magnus.ana@ki.se
Last application date15.Nov.2019 11:59 PM CET

Read the job description at the university homepage

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Post expires on Friday November 15th, 2019