Postdoc in Enzymology

A Postdoc position is available at the Section for ProteinChemistry & Enzyme Technology. Research in this section aims toattain new knowledge within protein- and enzyme-structure function relations,enzyme-substrate interactions, biocatalytic mechanisms, energy relations andkinetics, and on enzyme-protein robustness and stability.

The research will be conducted in the Interfacial Enzymology Group headedby prof. Peter Westh. The group investigates enzyme reactions that occur at theinterface of two immiscible phases; examples include the modification ofinsoluble substrate and activity of immobilized enzymes. We strive to providerigorous kinetic descriptions of such processes and to use kinetic andstructural knowledge in rational design of improved enzymes for industrialapplications.

Responsibilities and tasks 
The main theme of the work will be rational enzyme design. This willinvolve thorough kinetic characterization of enzyme activity on insolublesubstrate as well as analysis and mathematical modelling of the results. Theproject has special focus on relationships between substrate binding strengthand catalytic efficacy, and this includes analysis of so-called Linear FreeEnergy Relationships and application of the Sabatier Principle. Some relevantmodels for the analysis of kinetic data have already been developed within theInterfacial Enzymology group, but adaptions as well as implementation of novelmodel approaches may also be a part of the work. Key elements of the work include: 

    Experimental characterization of enzyme variants based on e.g.colorimetric, electrochemical and chromatographic methods.Combined analysis of kinetic and structural data for the purpose of elucidating molecular mechanisms and identifying beneficial mutations.Primary focus on cellulases acting on their natural, insoluble substrate cellulose, but work on other enzymes that modify insoluble substrate may be included.Identification of general traits of interfacial enzyme processes and the design of industrial enzymes.Protein expression including DNA manipulation, fermentation and purification.

The work will be conducted in close collaboration with experimentalistsand computational scientists at DTU Bioengineering and DTU Chemistry. As apostdoc, you will take part in the supervision of PhD students associated withthe project, and you will be committed to the integration of the project’sexperimental and computational activities. The project also involves closecollaboration with protein chemists and molecular biologists from Novozymes A/Sand applied mathematicians from Roskilde University. 

Qualifications
Candidates should have a PhD degree or equivalent within biochemistry,biophysics, biotechnology, protein chemistry or a related topic. We expect that the candidate: 

    Is interested and experienced in the kinetic description of enzyme reactions.Have the ability to motivate colleagues to be their best and achieve ambitious goals.Have extensive experience in enzymology including both the development and implementation of activity assays, and the stringent (model based) analysis of the results.Have been working with the structure and function of Glucoside Hydrolases.Have good qualifications in protein chemistry and general biophysics.Believe in dialogue and feedback as the best foundation for sound decision-making and scientific progress.Enjoy biochemical lab work and to develop and sophisticate biochemical protocols. 

We offer
DTU is a leading technical university globally recognized for theexcellence of its research, education, innovation and scientific advice. Weoffer a rewarding and challenging job in an international environment. Westrive for academic excellence in an environment characterized by collegialrespect and academic freedom tempered by responsibility.

Salary and terms of employment
The appointment will be based on the collective agreement with theDanish Confederation of Professional Associations. The allowance will be agreedupon with the relevant union. The post doc position is for 3 year.

The workplace is the DTU Campus in Lyngby, but minor activity in thelaboratories of Novozymes A/S should be expected. 

You can read more about career paths at DTU here.

Further information 
Further information may be obtained from Prof. Peter Westh, DTUBioengineering, petwe@dtu.dk ortel.: +45 4525 2641 or +45 3091 5488.

You can read more about DTU Bioengineering at www.bioengineering.dtu.dk/english and about the Section for Protein Chemistry & EnzymeTechnology atwww.bioengineering.dtu.dk/english/researchny/research-sections/section-for-protein-chemistry-and-enzyme-technology  

Application procedure
Please submit your online application no later than 20 January 2019 (local time). To apply, please open the link “Apply online”, fill out theonline application form. The following must be attached in English: 

    Application (cover letter)CVDiploma (MSc/PhD)List of publications 

Applications and enclosures received after the deadline will not beconsidered.

All interested candidates irrespective of age, gender, disability,race, religion or ethnic background are encouraged to apply.

DTU is a technical university providing internationally leadingresearch, education, innovation and scientific advice. Our staff of 6,000advance science and technology to create innovative solutions that meet thedemands of society, and our 11,200 students are being educated to address thetechnological challenges of the future. DTU is an independent academicuniversity collaborating globally with business, industry, government andpublic agencies.
Deadline: 20 January 2019
Unit: DTU Bioengineering
Read the job description and apply online

Post expires on Sunday January 20th, 2019